Ivis spectrum user manual

The ivis spectrum is the most advanced in vivo imaging system available on the market today not only can it quan titate and localize 3d fluorescent and bioluminescent sources in vivo, but it can import and automatically coregister ct or mri images yielding anatomical context. The ivis spectrum excitation and emission filters enable spectral. A long history in transplant medicine and now the era of regenerative medicine. Dw spectrum user interface ui includes the following main controls. Ivis lumina and ivis spectrumct unsw mark wainwright. Spectral unmixing not only allows detection and separation of. The ivis lumina xr should be operated only by personnel who have been trained in radiation safety and the operation and safety instructions contained in this manual.

E if you do not know what setting to use try 3060 second exposure time, large binning and fstop 1. Ivis filter recommendations f i lter recommendat i on s ivis spectrum, lumina ii and kinetic corporate headquarters 68 elm. The incredibly sensitive camera combined with high energy trans and epiillumination makes bioluminescence. If you have questions regarding this manual or the ivis imaging system, please.

Ivis filter recommendations ivis spectrum, lumina ii and. Welcome 2 the ivis spectrum is an integrated imaging system that includes a. The spectrum ct can quantitate bioluminescent or chemiluminescent signals produced by luciferases and other reporters, as. It is a vinnovafunded project to create basic conditions for the digitization of the swedish school, by building a completely open school administrative system. Inside the ivis lumina ii ccd camera the ivis lumina ii ccd is x mm square, with 1024 x 1024 pixels micron in width, yields higher imaging resolution backthinned, backilluminated grade 1 ccd provides high quantum efficiency over the entire visible to nearinfrared spectrum 16 bit digitizer delivers broad dynamic range. The xgi8 anesthesia module is the main device that controls the delivery of. The ivis spectrum preclinical optical imaging system combines high throughput and full tomographic capability for fluorescent and bioluminescent reporters. Bioluminescent and fluorescent imaging vectors cat. Based on our platform technology and experiences in the automotive field, ivis is growing up to the global leader in the future mobility industry. The rtsf ivis spectrum imaging core at michigan state university is a versatile, advanced in vivo bioluminescent and fluorescent preclinical. An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region.

We have 1 perkinelmer caliper ivis spectrum manual available for free pdf download. Canons advanced, highresolution hd cmos image sensor features extra sensitivity that delivers beautifully lifelike full hd video with natural color, even in low light. Please see the ivis spectrum ct hardware manual part no. The manual spectral unmixing option gives the user full control to separate out every component in the acquisition through the use of spectral subtraction and negative controls. Available filter pairs for ivis instrumentation filter recommendations for common fluorescent proteins, dyes and quantum dots using the ivis spectrum and lumina ii or ivis kinetic equipped with spectral unmixing filters.

The ivis lumina perkin elmer provides a highly sensitive means to image bioluminesence andor fluorescent reporters in vivo, ex vivo and in vitro. The ivis spectrum in vivo imaging system combines 2d optical and 3d optical tomography in one platform. Ivis spectrum in vivo imaging system university imaging. Description efficient gas delivery while minimizing excess gas exposure to lab personnel. The ivis website provides free access to original, uptodate publications organized in electronic books. The center for molecular and genomic imaging biomedical. Ivis spectrum ct offers bioluminescent, cerenkov and fluorescent imaging for true quantitative and calibrated analysis of optical data. Kinetic, please see the living image software users manual specific for the ivis kinetic. Perkin elmer caliper ivis spectrum in vivo system cell. Your email address will never be given to anyone outside of ivis. Perkinelmer caliper ivis spectrum hardware manual hardware manual 74 pages perkinelmer lambda 25.

Bioluminescence or fluorescence imaging 2d or 3d imaging modes. Caliper life sciences xenogen xgi8 community, manuals. Sign in page international veterinary information service. When generating an image series or imaging a group of animals, perform instrument subtraction first and only once. Up to 5 mice can be imaged simultaneously with a 23 cm field of view. The system uses leading optical technology for preclinical imaging research and development ideal for noninvasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals. The system enables simultaneous synchronization of functional and antomical data, coregistered for true quantitative 3d imaging. Perkinelmer spectrum two n user manual pdf download. International veterinary information service ivis is a notforprofit organization established to provide information to veterinarians, veterinary students, technicians and animal health professionals worldwide using internet technology. A biomedical cyclotron and radiochemistry laboratory allow onsite synthesis of custom radiotracers. Caliper ivis spectrum biological imaging development colab. Our global laboratories offer one of the worlds most comprehensive genomic screening and testing programs. Introducing perkinelmers ivis spectrum preclinical in vivo imaging system, the ultimate platform for quantitative bioluminescence and fluorescence imaging of whole animals to single cells.

Database contains 1 perkinelmer lambda 35 manuals available for free online viewing or downloading in pdf. Spectrum two n users guide about this manual this manual provides instructions for the installation, use and maintenance of the spectrum two n spectrometer. Ivis spectrum imaging research technology support facility. Perkinelmer caliper ivis spectrum hardware manual pdf download.

In vivo imaging has become a standard research tool in. Discovery in the living organism, ivis imaging system and living image are either registered trademarks or. Instrumentation resource facility school of medicine. Perkinelmer lambda 35 manuals and user guides, laboratory. Xenogens ivis 200 series and living image software. Animal imaging and preclinical testing 5916 ivis spectrum pre image preparation the instrument and computer should be left on all the times. Ivis spectrum in vivo imaging system university imaging centers. Perkinelmer uses cookies to ensure that we give you the best experience possible on our website. The ivis spectrum is the most versatile and advanced in vivo imaging system available. Home page international veterinary information service ivis. Caliper life sciences xenogen xgi8efficient gas delivery while minimizing excess gas exposure to lab personnel. Ccd camera mounted on a lighttight imaging chamber camera power supply thermoelectric cooling unit fluorescence light source windowsbased computer system for data acquisition and analysis. If you have questions regarding this manual or the ivis.

Ivis lumina ii cls5289 ivis lumina xr cls5290 ivis kinetic cls5288 ivis 200 cls5287. This user manual explains how to acquire optical image data on an ivis imaging system. Each user will log on to ivis instruments using the kerberos username and password. The small animal imaging facility located in the absl3 facility of the vivarium in the saint louis university doisy research center offers sophisticated equipment for the optical imaging of small animals. If you have questions regarding this manual or the ivis lumina xr, please contact. Ivis ags 531 75701 edition 052011 5 1 safety provisions the work described in this manual may only be performed by specialist electricians who have proved their experience with the ivis f display and the applicable safety. The ivis spectrum ct automates the complete workflow from image acquisition and re. The manual provides detailed instructions and screenshots that depict the. User manual a limiteduse label license covers this product. By use of this product, you accept the terms and conditions outlined in the licensing and warranty statement contained in this user manual. The ivis lumina takes very low light level images that can be saved and displayed for subsequent analysis.

The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use r ealtime in vivo imaging to monitor and record cellular and genetic activity within a living organism. Right now we have only cybersecurity and we can quickly see that we have 24 completed, 15 controls with a poam, 17. With exquisitely sensitive and unique optical detection technology and spectral unmixing, the ivis spectrum ct can identify very small optical sources within an animal. Ivis fluorescent imaging guide university of chicago. The ivis spectrum is a wholebody optical imaging system featuring highsensitivity in vivo imaging of fluorescence and bioluminescence. It contains main menu, tabsand the following buttons. Ivis spectrum ivis spectrum in vivo imaging system uses optical imaging technology to facilitate noninvasive monitoring of bioluminescent and fluorescent reporters in living animals. Ivis spectrumct in vivo imaging system perkinelmer. Cls5292 is only for use with the ivis spectrum ct instrument. Automatically archive camera information and userspecified annotations with each image. The ivis spectrum, new in 2015, is comprised of a sensitive ccd camera, a dark imaging chamber to minimize incident light, and specialized software to quantify and analyze results. We are developing embedded software for various device systems like infotainment system or digital instrument cluster.

This user manual explains how to acquire optical and volumetric image data on the ivis spectrum and analyze the data using the living image software. Ivis spectrum ct living image software simplifies imaging calipers living image software is designed to simplify advanced and complex biological models by intuitively guiding the user in experimental setup and analysis. The spectrum ct can quantitate bioluminescent or chemiluminescent signals produced by. This button indicates the current connection status and allows to connect or disconnect. The ivis controls dashboard gives you a quick overview of all core elements you have installed in your instance. Epiillumination is the standard 2d image that is covered in basic training. The system provides researchers with greater insights into complex biological systems by enabling simultaneous molecular and anatomical noninvasive imaging. The ivis spectrum is versatile and has the capability to use either transillumination from the bottom or epiillumination from the top to. Log in by selecting your initials from the drop down menu, or if you are a new user, enter your initials and click done. Home page international veterinary information service. The ivis spectrum is the most versatile and advanced in vivo imaging system available on the market today.

System manual ivis lumina xr realtime bioluminescence. The rtsf ivis spectrum imaging core at michigan state university is a versatile, advanced in vivo bioluminescent and fluorescent preclinical imaging system that combines highthroughput and full tomographic optical imaging in one platform. The ivis website is a professional information resource reserved to veterinarians, veterinary support staff, veterinary students, and animal health scientists and professionals. Turn on the evacuation pump using the switch on the front panel. If you have questions regarding this manual or the ivis lumina, please. In addition to bioluminescent imaging, the ivis lumina incorporates the xfo12 fluorescence equipment that enables fluorescent imaging capabilities for both in. For advanced fluorescence preclinical imaging, the ivis spectrum has the capability to use either transillumination from the bottom or epiillumination from the top to illuminate in vivo fluorescent sources. Bioluminescence imaging bli of transgenic animals or cells expressing luciferase requires the administration of luciferin, a bioluminescent substrate, prior to imaging. It has applications in many fields of research such as oncology, stem cell research, infectious disease.

Ivis spectrum user guide booking reservation for the ivis spectrum must be done through the nu core scheduler. Perkinelmer caliper ivis spectrum manuals manuals and user guides for perkinelmer caliper ivis spectrum. Our users transition from ivis to lago was seamless. Perkinelmer caliper ivis spectrum hardware manual pdf. For instructions on using the system software, please see the living image. In vivo imaging core facility vcr university of nebraska. The xgi8 anesthesia system is used in conjunction with the ivis spectrum to anesthetize mice undergoing bioluminescent or fluorescent whole body imaging. User manuals, guides and specifications for your perkinelmer lambda 35 laboratory equipment. The ivis spectrum can image and quantify all commonly used fluorophores, including fluorescent proteins, dyes and conjugates. Feel free to contact the core manager with other animal questions.

The ivis spectrum ct maintains the versatility and the most. The center for molecular and genomic imaging is a fullyequipped core facility that provides in vivo animal imaging and biospecimen imaging. Ivis spectrum 4 multimodality coregistration fluorescence ct coregistration analysis the ivis spectrum is the most advanced in vivo imaging system available on the market today not only can it quantitate and localize 3d fluorescent and bioluminescent sources in vivo, but it can import and automatically coregister. Ivis is automotive embedded software development company. Ivis spectrum advanced optical microscopy facility aomf. Introduction warnings and safety information an overview of the spectrum two n. Oxygen tank is provided by cam and connected with anesthesia machine and if the. Start the anesthesia system all users must been trained on impac 6 by ccm prior to ivis spectrum training.

No need to confine or morph your subject, structured light measure. Noninvasive in vivo imaging allows researchers to sequentially image animals over time yielding valuable information on disease progression, cell trafficking and gene expression patterns in living animals. Perkinelmer ivis spectrum ct biophotonic imager the perkinelmer ivis spectrum ct biophotonic imager excels at imaging light emitted from small animals such as mice or rats but can also be used to image light emitted from western blots, culture dishes and 96well plates. For instructions on using the system software, please see the living image software users manual. The spectrum achieves superior spectral unmixing through a wide range of high resolution, short cutoff filters and advanced spectral unmixing algorithms. The soundlimiting enclosure beneath the imaging chamber contains the. The center for molecular imaging ivis spectrum bioluminescence protocol 4 while you wait the 10 minutes set luminescent imaging mode, exposure time, binning, and fstop to the right level. Spectral instruments imaging in vivo imaging instruments.